Microsoft Word - 57260759-file00

1 Adaptive evolution of fish hatching enzyme: 1 One amino acid substitution results in differential salt dependency of the enzyme 2 3 Mari Kawaguchi , Shigeki Yasumasu, Akio Shimizu, Norio Kudo, Kaori Sano, 4 Ichiro Iuchi and Mutsumi Nishida 5 Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, 6 Kashiwa, Chiba 277-8564, Japan 7 Research Fellow of the Japan Society for the Promotion of Science (JSPS), Japan 8 Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia 9 University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan 10 National Research Institute of Fisheries Science, Fisheries Research Agency, Kanazawa, 11 Yokohama 236-8648, Japan 12 Seed Compounds Exploratory Unit for Drug Discovery Platform, RIKEN Advanced Science 13 Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan 14 15 Abbreviated Title: Environmental adaptation of hatching enzyme 16 17 Corresponding Author: Mutsumi Nishida 18 Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, 19 Kashiwa, Chiba 277-8564, Japan 20 Tel: +81-4-7136-6210 Fax: +81-4-7136-6211 21 E-mail: [email protected] 22 *Present address: Department of Materials and Life Sciences, Faculty of Science and 23 Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan 24 25 26 http://jeb.biologists.org/lookup/doi/10.1242/jeb.069716 Access the most recent version at J Exp Biol Advance Online Articles. First posted online on 24 January 2013 as doi:10.1242/jeb.069716